Nucleic acid construct, kit, detection method, and therapeutic effect prediction method
AssigneeKabushiki Kaisha Toshiba
InventorsShigehisa Kawata, Mitsuko Ishihara
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Abstract
According to one embodiment, a nucleic acid construct is used for detecting an estrogen-sensitive cell. The nucleic acid construct includes an enhancer sequence, a promoter sequence and a reporter gene. The enhancer sequence includes at least an ERE sequence and at least a XRE sequence. The promoter sequence is ligated to downstream of the enhancer sequence. The reporter gene is ligated to downstream of the promoter sequence.
Claims23
A nucleic acid construct for detecting an estrogen-sensitive cell, comprising:
- an enhancer sequence including at least an ERE sequence and at least a XRE sequence;
- a promoter sequence ligated to downstream of the enhancer sequence;
- and
- a reporter gene ligated to downstream of the promoter sequence.
A kit for detecting estrogen-sensitive cells, comprising a nucleic acid construct encapsulated in a lipid particle, the nucleic acid construct including:
- an enhancer sequence including at least an ERE sequence and at least an XRE sequence;
- a promoter sequence ligated to downstream of the enhancer sequence;
- and
- a reporter gene ligated to downstream of the promoter sequence.
A method for detecting an estrogen-sensitive cell in a test cell by using a nucleic acid construct, the nucleic acid construct including:
- an enhancer sequence including at least an ERE sequence and at least a XRE sequence;
- a promoter sequence ligated to downstream of the enhancer sequence;
- and
- a reporter gene ligated to downstream of the promoter sequence,
- the detection method comprising:
- introducing the nucleic acid construct into the test cell;
- adding estrogen to the test cell;
- culturing the test cell;
- detecting an activity of a reporter protein expressed from the reporter gene;
- and
- determining the estrogen-sensitive cell from a result of the detection.
A method for predicting efficacy of hormone treatment for cancer in a subject, by using a nucleic acid construct, the nucleic acid construct including:
- an enhancer sequence including at least an ERE sequence and at least a XRE sequence;
- a promoter sequence ligated to downstream of the enhancer sequence;
- and
- a reporter gene ligated to downstream of the promoter sequence,
- the method comprising:
- introducing the nucleic acid construct into a test cell obtained from the subject;
- adding estrogen to the test cell;
- culturing the test cell;
- detecting activity of a reporter protein expressed from the reporter gene;
- and
- predicting efficacy of hormone treatment for cancer in the subject from a result of the detection.
Dependencies are inferred from the claim text.