Markers specific for pluripotent stem cells, and methods of using the same
AssigneeFUJIFILM Corporation
InventorsThomas Burke, Steven Smith, Anne Strouse
Life of this patent
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Abstract
The present disclosure provides markers specific for pluripotent stem cells. In particular, the present disclosure relates to nucleic acid and polypeptide markers that are selectively expressed by pluripotent stem cells; and to methods for detecting the presence and/or absence of one or a plurality of pluripotent stem cells, by detecting such markers.
Claims25
A method for detecting pluripotent stem cells, said method comprising:
- (a) obtaining a sample of interest containing a plurality of cells;
- (b) determining that the sample of interest does not contain pluripotent stem cells by analyzing said sample of interest to detect n expression of at least one transcript having a corresponding complementary DNA (cDNA) sequence that comprises a nucleotide sequence having at least 90% sequence identity to a full length of a nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 1-17 and 19-28, wherein it is determined that the sample of interest does not contain pluripotent stem cells when the expression of said at least one transcript is not detected, and
- (c) producing a product containing differentiated cells using the sample of interest determined not to contain pluripotent stem cells.
A method for detecting pluripotent stem cells, said method comprising:
- (a) obtaining a sample of interest containing a plurality of cells;
- (b) determining that the sample of interest contains substantially no pluripotent stem cells by (b)(1) measuring, in said sample of interest, an expression level of at least one transcript having a corresponding complementary DNA (cDNA) sequence that comprises a nucleotide sequence having at least 90% sequence identity to a full length of a nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 1-17 and 19-28, and
- (b)(2) measuring the expression level of said at least one transcript in at least one reference sample, wherein said at least one reference sample comprises a plurality of cells, and wherein said at least one reference sample contains substantially no pluripotent stem cells,
- wherein it is determined that the sample of interest contains substantially no pluripotent stem cells when the expression level detected in step (b)(1) is equal to or less than the expression level measured in step (b)(2);
- and
- (c) producing a product containing differentiated cells using the sample of interest determined to contain substantially no pluripotent stem cells.
A method for quantifying the number of pluripotent stem cells in a product containing differentiated cells, said method comprising:
- (a) obtaining the product containing differentiated cells;
- (b) measuring, in said product containing differentiated cells, a level of expression of at least one transcript having a corresponding complementary DNA (cDNA) sequence that comprises a nucleotide sequence having at least 90% sequence identity to a full length of a nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 1-17 and 19-28;
- and
- (c) measuring a level of expression of said at least one transcript in at least one reference sample, wherein said at least one reference sample comprises a plurality of cells, wherein said at least one reference sample comprises pluripotent stem cells, and wherein substantially all of the cells in said at least one reference sample are pluripotent stem cells.
Dependencies are inferred from the claim text.