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US 12,571,031 B2Granted
Nucleic acid modifying reagents and uses thereof
AssigneePromega Corporation
InventorsCe Shi, Nathan Feirer, Michael A. Scurria, Subhanjan Mondal, Said A. Goueli, Thomas Kirkland, James J. Cali, Poncho Meisenheimer
Life of this patent
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Abstract
The present disclosure includes compounds, compositions, and methods for nucleic acid amplification reactions. In particular, the present disclosure provides compounds, compositions, and methods for viability PCR (vPCR) applications, in which the compounds that selectively bind to nucleic acids from non-viable cells.
Claims13
A compound or a salt thereof, the compound comprising:
- (A) a nucleic acid binding moiety (“NAB moiety”) having a structure selected from:
- (B) a live/dead cell differentiating moiety (“LDCD moiety”) having at least one charged moiety;
- and
- (C) a nucleic acid modifying moiety (“NAM moiety”) having a structure selected from:
1 more element
A compound selected from:
- and a salt of any thereof.
Dependencies are inferred from the claim text.