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US 12,571,022 B2Granted
Methods for quantifying mixed-linkage beta glucan
AssigneeEdeniq, Inc.
InventorsPrachand Shrestha, Denmark Antolin
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Abstract
Methods and solutions are disclosed for the quantification of mixed-linkage beta-glucan in plant biomass samples. Improved alkaline pretreatment, enzymatic hydrolysis, and glucose measurement parameters are provided that increase the accuracy, precision, and sensitivity of mixed-linkage beta-glucan assays.
Claims19
A method of accurately quantifying a concentration of mixed-linkage beta-glucan in a plant biomass sample, the method comprising:
- contacting the plant biomass sample with an alkaline mixture thereby generating a pretreated sample, wherein the alkaline mixture comprises a metal hydroxide at a concentration between 0.2 N and 1 N, the metal hydroxide comprising sodium hydroxide or potassium hydroxide;
- hydrolyzing the mixed-linkage beta-glucan in the pretreated sample to generate glucose;
- measuring the concentration of glucose in the pretreated sample;
- and
- transforming the measured glucose concentration to a calculated mixed-linkage beta-glucan concentration by multiplying the measured glucose concentration by the theoretical yield of glucose from mixed-linkage beta-glucan via hydrolysis, wherein the calculated mixed-linkage beta-glucan concentration accurately quantifies the concentration of mixed-linkage beta-glucan in the plant biomass sample.
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Dependencies are inferred from the claim text.