Method for producing composition containing polypeptide with suppressed coloring
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsTakahiro Matsuo, Masao Mori, Atsushi Kunimune
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Abstract
Provided is a method for producing a composition containing a polypeptide with suppressed coloring, the method comprising the steps of (a) culturing eukaryotic cells containing a nucleic acid encoding a polypeptide in a cell culture medium free of vitamin B12; and (b) collecting a composition containing the polypeptide from the culture.
Claims17
A method for producing a composition containing a polypeptide with suppressed coloring, wherein the polypeptide is a Fc-containing polypeptide, the method comprising the steps of:
- a) culturing CHO cells containing a nucleic acid encoding the Fc-containing polypeptide by fed-batch culture in a cell culture medium, wherein the fed-batch culture is performed with an initial culture medium and a feed culture medium wherein the concentration of cobalt molecules contained in vitamin B12 in both culture media is less than 20 ppb;
- and
- b) collecting a composition containing the polypeptide from the culture.
A method for producing a composition containing an antibody with suppressed coloring, the method comprising the steps of:
- a) identifying an antibody of interest as an antibody containing a modification of at least one amino acid residue selected from amino acid residues at positions 214, 234, 238, 250, 264, 307, 311, 330, 343, 428, 434, 436, 438 and 440 according to EU numbering in the Fc region;
- b) selecting a cell culture medium for the antibody containing a modification of the amino acid residue, wherein the concentration of cobalt molecules contained in vitamin B12 in the culture medium is less than 20 ppb;
- c) culturing CHO cells containing a nucleic acid encoding the antibody containing a modification of the amino acid residue in the cell culture medium selected in step b) by fed-batch culture;
- and
- d) collecting a composition containing the antibody from the culture.
Dependencies are inferred from the claim text.