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US 12,571,011 B2Granted
Viscous polyhydroxyalkanoate, preparation thereof, and use thereof
AssigneeSouth China University of Technology
InventorsJianwen Ye, Yina Lin, Xinying Xie
Life of this patent
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Abstract
The present application relates to the field of microbiological technology, and particularly relates to a viscous polyhydroxyalkanoate, and its preparation and use. The viscous polyhydroxyalkanoate can be produced by fermenting a Pseudomonas strain containing a mutant of PHA polymerase PhaC61-3, and can be used for the preparation of biodegradable products.
Claims10
A variant of the polypeptide of SEQ ID NO:
- 9,
- wherein the variant has polyhydroxyalkanoate polymerase activity, and the variant comprises all of SEQ ID NO:
- 9 except for:
- a T at the position corresponding to position 115 of SEQ ID NO:
- 9;
- an E at the position corresponding to position 261 of SEQ ID NO:
- 9;
- an S at the position corresponding to position 360 of SEQ ID NO:
- 9;
- and
- a Q at the position corresponding to position 457 of SEQ ID NO:
- 9.
9 more elements
A nucleic acid encoding a variant of the polypeptide of SEQ ID NO:
- 9,
- wherein the variant has polyhydroxyalkanoate polymerase activity, and the variant comprises all of SEQ ID NO:
- 9 except for:
- a T at the position corresponding to position 115 of SEQ ID NO:
- 9;
- an E at the position corresponding to position 261 of SEQ ID NO:
- 9;
- an S at the position corresponding to position 360 of SEQ ID NO:
- 9;
- and
- a Q at the position corresponding to position 457 of SEQ ID NO:
- 9.
9 more elements
A nucleic acid encoding a variant of the polypeptide of SEQ ID NO:
- 9, wherein the variant has polyhydroxyalkanoate polymerase activity and comprises the amino acid sequence of SEQ ID NO:
- 10.
A variant of the polypeptide of SEQ ID NO:
- 9, wherein the variant has polyhydroxyalkanoate polymerase activity and comprises the amino acid sequence of SEQ ID NO:
- 10.
Dependencies are inferred from the claim text.