US 12,571,001 B2 · Baculovirus vector and use thereof in preparation of recombinant adeno-associated virus (rAAV) in insect cell

Abstract

A baculovirus vector and a use thereof in the preparation of a recombinant adeno-associated virus (rAAV) in an insect cell are provided. The baculovirus vector includes an exogenous gene expression cassette and a stable sequence. The stable sequence is located at a site 5 kb or less from the exogenous gene expression cassette, and the stable sequence is a conserved noncoding element (CNE) sequence or a nucleocapsid assembly-essential element (NAE) sequence. When an insect cell is infected with a recombinant baculovirus (rBV) constructed in this way, after multiple continuous passages, production levels of the rBV and the rAAV still remain relatively stable.

Claims17

Dependent claims

A recombinant baculovirus (rBV) shuttle vector for preparing a rBV, comprising an exogenous gene expression cassette and a stable sequence, wherein the stable sequence is a conserved noncoding element (CNE) sequence or a nucleocapsid assembly-essential element (NAE) sequence;

the exogenous gene expression cassette is at least one of a cap gene expression cassette of an adeno-associated virus (AAV), a rep gene expression cassette of the AAV, and an AAV inverted terminal repeat (ITR) core expression element operably linked to a heterologous coding sequence;
and the stable sequence is located at a site 5 kb or less from at least one of a start and a stop end of the cap gene expression cassette, a start and a stop end of the rep gene expression cassette, and two ends of the AAV ITR core expression element operably linked to the heterologous coding sequence.

Dependencies are inferred from the claim text.

Baculovirus vector and use thereof in preparation of recombinant adeno-associated virus (rAAV) in insect cell

Life of this patent

Baculovirus vector and use thereof in preparation of recombinant adeno-associated virus (rAAV) in insect cell

Life of this patent

Abstract

Claims17