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US 12,570,987 B2Granted
Synthetically evolved DNA constructs for regulating signal peptide performance as well as vectors, host cells and recombinant proteins thereof
AssigneeCloneopt AB
InventorsKiavash Mirzadeh, Daniel Daley, Patrick Shilling
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Abstract
The present invention provides a simple and inexpensive system for regulating signal peptide performance by using a synthetically evolved nucleotide sequence. The invention further relates to an expression vector comprising the nucleotide sequence. Additionally, the present invention relates to host cell comprising the expression vector. Furthermore, the present invention relates to a recombinant protein expressed by the host cell as well as a method for expressing the recombinant protein.
Claims14
A DNA construct suitable for regulating signal peptide performance, wherein said DNA construct comprises:
- a. a Shine-Dalgarno sequence;
- b. an ATG start codon;
- c. a sequence of one of SEQ ID 1-28 comprising said ATG start codon;
- and
- d. a signal peptide encoding sequence,
- wherein said sequence of one of SEQ ID 1-28 comprises at least the first 9 nucleotides of said signal peptide encoding sequence;
- wherein said sequence of one of SEQ ID NO 15-28 comprises said Shine-Dalgarno sequence, said ATG start codon and said sequence of one of SEQ ID 1-14.
4 more elements
Dependencies are inferred from the claim text.