Compositions and methods for inhibiting intermediate filament tetramerization
AssigneeYale University
InventorsChristopher Bunick, Sherif Eldirany, Minh Ho
Life of this patent
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Abstract
The present invention provides compositions and methods for inhibiting intermediate filament tetramerization and formation.
Claims2
A method of screening a library of compounds to provide an inhibitor of intermediate filament formation, wherein the inhibitor inhibits the interaction between an anchoring knob domain in segment 1B of a first intermediate filament dimer and a hydrophobic pocket domain in segment 1B of a second intermediate filament dimer, the method comprising:
- (a) contacting a hydrophobic pocket domain in segment 1B of an intermediate filament dimer with at least one compound in the library of compounds;
- (b) measuring a binding of the at least one compound to the hydrophobic pocket domain in segment 1B;
- (c) comparing the binding of the at least one compound to the hydrophobic pocket domain in segment 1B, with a binding of a comparator control to the hydrophobic pocket domain in segment 1B;
- and
- (d) selecting the at least one compound from the library when the binding of the at least one compound to the hydrophobic pocket domain in segment 1B, is increased at a statistically significant amount when compared with.
A method of screening a library of compounds to provide an inhibitor of intermediate filament tetramer formation, wherein the inhibitor inhibits the interaction between an anchoring knob domain in segment 1B of a first intermediate filament dimer and a hydrophobic pocket domain in segment 1B of a second intermediate filament dimer, the method comprising:
- (a) mixing a first intermediate filament dimer with at least one compound from the library to form a mixture;
- (b) adding a second intermediate filament dimer to the mixture;
- (c) measuring intermediate filament formation;
- (d) comparing the tetramer formation to a comparator control;
- and
- (e) selecting the at least one compound from the library when the tetramer formation is altered at a statistically significant amount when compared with the tetramer formation of the comparator control.
Dependencies are inferred from the claim text.