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AssigneeYeda Research and Development Co. Ltd.
InventorsYaqub Hanna, Noa Novershtern, Yoach Rais
Estimating the term…
Provided is an isolated human naive pluripotent stem cell (PSC), wherein: (i) when the naive PSC is a female PSC, then said naive female PSC has two unmethylated alleles of an X-inactive specific transcript (XIST) gene; and (ii) when said naive PSC is a male PSC, then said naive male PSC has an unmethylated allele of said XIST gene. Also provided is a culture medium which comprises an ERK1/2 inhibitor, a GSK3beta inhibitor, a p38 inhibitor, a JNK inhibitor, a STAT3 activator and at least one agent selected from the group consisting of: bFGF, TGFbeta 1, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor; or at least agent selected from the group consisting of: a TGFR inhibitor, a FGFR inhibitor, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor.
A culture medium comprising an ERK1/2 inhibitor at a concentration of 0.3-1.5 μM, a GSK3β inhibitor at a concentration of 0.1-0.5 μM, a p38 inhibitor at a concentration of 0.1-2 μM, a JNK inhibitor at a concentration of 0.5-5 μM, a STAT3 activator at a concentration of 5-50 ng/ml and at least one agent selected from the group consisting of:
A culture medium comprising an ERK1/2 inhibitor at a concentration of 0.3-1.5 μM, a GSK3β inhibitor at a concentration of 0.1-0.5 μM, a p38 inhibitor at a concentration of 0.1-2 μM, a JNK inhibitor at a concentration of 0.5-25 μM, a STAT3 activator at a concentration of 5-50 ng/ml and at least one agent selected from the group consisting of:
Dependencies are inferred from the claim text.