Systems and methods for processing tissue
AssigneeAnnoviant, Inc.
InventorsNarendra R. Vyavahare, Teodor Dan Simionescu, Agnes Nagy-Mehesz, Ajay Houde
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Abstract
Embodiments of the present specification provide systems and methods for holding one or more tissues, such as veins, such that the tissue remains open in a chamber while undergoing orbital shaking with various solutions and to allow uniform treatment during a decellularization process. A frame is held on a stand to which the tissues are attached and comprises a tension inducing mechanism to cause the tissues to controllably stretch. The frame is removed from the stand, with the tissue attached to it, and placed in a decellularization chamber for uniform treatment of the tissue.
Claims25
A processing system adapted to decellularize one or more pieces of biological tissue having a cylindrical shape, comprising:
- a processing device comprising:
- a frame configured to hold the one or more pieces of biological tissue, wherein each of the one or more pieces of biological tissue comprises a first end defined by a first circular periphery, a second end, opposing the first end, defined by a second circular periphery, and a length defined by a longitudinal axis that extends between the first end and the second end;
- a first holder comprising a first ring configured to atraumatically attach to the first circular periphery of at least one of the one or more pieces of biological tissue;
- a second holder comprising a second ring configured to atraumatically attach to the second circular periphery of at least one of the one or more pieces of biological tissue;
- a first member configured to attach the first holder to the frame;
- and
- a second member configured to attach the second holder to the frame, wherein at least one of the first member or second member is configured to lengthen or contract so as to apply tension along the longitudinal axis of the at least one of the one or more pieces of biological tissue to preserve its cylindrical shape;
- a chamber, wherein the processing device is configured to fit within an internal volume of the chamber;
- and
- a decellularization solution positioned within the internal volume of the chamber.
Dependencies are inferred from the claim text.