US 12,570,944 B2Granted

Devices and methods for magnetic isolation and analysis of rare cells

AssigneeThe Johns Hopkins University

InventorsDan Stoianovici, Kenneth J. Pienta, Michael A. Gorin

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Abstract

An embodiment in accordance with the present invention provides two devices for the isolation of rare cell populations such as circulating tumor cells (CTCs). Both devices use magnetic fields to manipulate cells that are bounded to paramagnetic particles (PMP). One device uses surface tension and a sieve for cell filtration, whereas the other allows for the direct transfer of cells onto a standard microscope slide. Subsequent processing steps may be directly performed on the devices thereby minimizing cell losses. The first device is referred to as the ST (surface tension) device and the second device is referred to as the DT (direct transfer) device. The ST device can also be considered as a chip for the isolation of the rare cell populations.

Claims5

Dependent claims

A device for isolation of a rare cell population comprising:

a direct transfer device configured for isolating the rare cell population having, an input well for receiving a solution containing the rare cell population;
a collecting surface configured for collecting the rare cell population from the solution received in the input well, wherein the collecting surface takes the form of a microscope slide having a first face and a second face opposite the first face, and wherein a first face of the collecting surface is releasably couplable to the input well;
and,
a magnetic source holder cavity, wherein the magnetic source holder cavity is configured to keep a magnetic source in contact with the second face of the collecting surface to affect transfer of the rare cell population from the solution containing the rare cell population to the collecting surface.

1 more element

Dependencies are inferred from the claim text.

Facts

Application: 17/466,654Filed 3 Sep 2021 · continuation
Priority: 21 Apr 2016
Published: US 2021/0395666 A123 Dec 2021
Granted: 10 Mar 2026
Term ends: Not estimatedThe source records don’t support an estimate.

Classification24 codes

C12M 33/14
Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus: with…
B01L 3/502
Containers for the purpose of retaining a material to be analysed, e.g. test tubes: with fluid transport, e.g. in multi-compartment structures
B01L 3/508
Containers for the purpose of retaining a material to be analysed, e.g. test tubes: rigid containers without fluid transport within
B01L 3/5635
Joints or fittings; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors: connecting two containers…

Family

Application62/325,629Us provisional application · filed 21 Apr 2016
PCT/US2017/028746Parent of this continuation · filed 21 Apr 2017
US 2021/0395666 A1Pre-grant publication · published 23 Dec 2021
Application16/095,505Parent of this continuation
US 2019/0127680 A1Published application
US 12,570,944 B2This patent

From the US filing history. Foreign family members aren’t listed.