Method for preparing chemiluminescent hydrogel

A method for preparing a chemiluminescent hydrogel, comprising the following steps:

• step 1, adding chitosan to an acetic acid solution, and stirring until the chitosan is completely dissolved;
• wherein the acetic acid solution has a volume fraction of 1.0-10.0%, and the chitosan and the acetic acid solution have a ratio of (0.5-1.5) g:(15.0-25.0) mL;
• step 2, adding a cobalt chloride solution and a polyvinyl alcohol (PVA) solution to a product obtained in step 1, stirring evenly, and ultrasonically defoaming;
• wherein the cobalt chloride solution has a concentration of 15-60 mmol/L, the PVA solution has a mass fraction of 5.0-10.0%, and the acetic acid solution, the cobalt chloride solution and the PVA solution have a volume ratio of (15.0-25.0):(0.5-2.0):(0-10.0);
• step 3, soaking a mixture obtained in step 2 in an alkaline solution for alkaline bath treatment, and letting the mixture stand overnight to obtain a hydrogel;
• wherein the alkaline solution has a concentration of 1.0-10.0 mol/L;
• and
• step 4, mixing a product obtained in step 3 with an N-(4-aminobutyl)-N-ethylisoluminol solution, stirring evenly, adding a resulting mixture to a hydrogen peroxide solution to obtain the chemiluminescent hydrogel;
• wherein the N-(4-aminobutyl)-N-ethylisoluminol solution has a concentration of 1-24 mmol/L, the hydrogen peroxide solution has a concentration of 0.01-1.00 mol/L, and the product obtained in step 3, the N-(4-aminobutyl)-N-ethylisoluminol solution, and the hydrogen peroxide solution have a volume ratio of (1-3):(1-3):(2-6).