Hydrogel-free surface functionalization for sequencing
AssigneeIllumina, Inc.
InventorsXiaoyu Ma, Weixian Xi, Jeffrey S. Fisher, Fei Shen, Wayne N. George, Brian D. Mather
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Abstract
Embodiments of the present application relate to substrate comprising a surface-bound azido functionalized organosilane wherein the substrate is free or substantially free of a hydrogel or a hydrophilic polymer. Methods of preparing such substrate surface for sequencing applications are also disclosed.
Claims23
A substrate comprising a surface-bound organosilane covalently attached to a plurality of biological molecules, wherein the surface-bound organosilane comprises azido groups that are covalent bonded with the plurality of biological molecules, each azido group is attached to a silicon atom of the organosilane through a linker moiety, and wherein the substrate is free or substantially free of a hydrogel or a hydrophilic polymer.
A method of functionalizing a surface of a substrate, comprising:
- depositing an organosilane to the surface of the substrate to form a surface-bound organosilane layer, wherein the surface-bound organosilane layer comprises azido groups, each azido group is attached to a silicon atom of the organosilane through a linker moiety, and wherein the method does not comprise depositing a hydrogel or a hydrophilic polymer to the surface of the substrate prior to or after the deposition of the organosilane.
A method of immobilizing oligonucleotides to a substrate, comprising:
- contacting a plurality of oligonucleotides with an organosilane bound surface of the substrate, wherein the organosilane comprises azido groups, each azido group is attached to a silicon atom of the organosilane through a linker moiety, and wherein the substrate is free or substantially free of a hydrogel or a hydrophilic polymer;
- and
- reacting the plurality of the oligonucleotides with the azido groups of the organosilane to covalently attach the oligonucleotides to the substrate.
Dependencies are inferred from the claim text.