Recombinant humanized collagen type III alpha-1, and expression vector and use thereof
AssigneeShandong D-Nutrimec Biomedical Co., Ltd.
InventorsZhidong Guo, Jiajia Shao, Hongwei Ma
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AssigneeShandong D-Nutrimec Biomedical Co., Ltd.
InventorsZhidong Guo, Jiajia Shao, Hongwei Ma
Estimating the term…
A recombinant humanized collagen type III alpha-1 (rhCol III α1), and an expression vector and use thereof are provided, relating to the technical field of bioengineering. A sequence on positions 154 to 1,221 of a human type III collagen α chain is ligated to an optimized vector pcDNA3.1, a resulting optimized expression vector is transfected into a human embryonic kidney cell Expi293F, and then rhCol III α1 is secreted to form a fusion protein with a soluble green fluorescent protein (GFP). The expressed rhCol III α1 that forms the fusion protein with the GFP shows an activity of promoting the migration of BALB/c 3T3 cells, thus exhibiting a great application potential in the field of skin care.
A recombinant humanized collagen type III alpha-1 (rhCol III α1) fusion protein consisting of SEQ ID NO:
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