Methods for detecting intercellular interactions of cells in a compartment of a fluidic device
AssigneeCellanome, Inc.
InventorsTarun Kumar Khurana, Ali Agah, Yir-Shyuan Wu, Filiz Gorpe Yasar
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Abstract
Described herein are systems and methods for analyzing biological samples. The methods can include forming a plurality of compartments, wherein a compartment of the plurality of compartments may enclose a cell inside of a fluidic device. The methods can further include detecting an interaction between the cell and an additional cell within the fluidic device.
Claims24
A method for analyzing an intercellular interaction, comprising:
- a) providing energy to a fluidic device comprising a fluid to polymerize a polymer precursor in the fluidic device, thereby forming a plurality of compartments comprising a polymer matrix wall within the fluid, wherein the fluid comprises a plurality of cells, wherein a first cell of the plurality of cells is enclosed in a compartment of the plurality of compartments;
- b) detecting the intercellular interaction between the first cell and a second cell of the plurality of cells;
- and
- c) analyzing a nucleic acid from the first cell.
A method for analyzing an intercellular interaction, comprising:
- a) providing energy to a fluidic device comprising a fluid to polymerize a polymer precursor in the fluidic device, thereby forming a plurality of compartments comprising a polymer matrix wall within the fluid, wherein the fluid comprises a plurality of cells, wherein a first cell of the plurality of cells is enclosed in a compartment of the plurality of compartments, and wherein the first cell secretes a protein;
- b) detecting the intercellular interaction between the first cell and a second cell of the plurality of cells;
- c) capturing the protein on a capture antibody, wherein the capture antibody is inside of the compartment;
- d) coupling a detection antibody to the protein;
- and
- e) detecting the detection antibody.
A method for analyzing an intercellular interaction, comprising:
- a) providing energy to a fluidic device comprising a fluid to polymerize a polymer precursor in the fluidic device, thereby forming a plurality of compartments comprising a polymer matrix wall within the fluid, wherein the fluid comprises a plurality of cells, wherein a first cell of the plurality of cells is enclosed in a compartment of the plurality of compartments with antibodies specific for surface proteins;
- b) detecting the intercellular interaction between the first cell and a second cell of the plurality of cells;
- and
- c) determining a relative expression of the surface proteins on the first cell.
A method for analyzing an intercellular interaction, comprising:
- a) providing energy to a fluidic device comprising a fluid to polymerize a polymer precursor in the fluidic device, thereby forming a plurality of compartments comprising a polymer matrix wall within the fluid, wherein the fluid comprises a plurality of cells, wherein a first cell of the plurality of cells is enclosed in a compartment of the plurality of compartments;
- and
- b) detecting the intercellular interaction between the first cell and a second cell of the plurality of cells,
- wherein the fluid is in contact with a surface of the fluidic device, wherein the surface comprises a cell capture element, and wherein subsequent to a), the first cell, the second cell, or the first cell and the second cell are coupled to the cell capture element.
Dependencies are inferred from the claim text.