De novo synthesized gene libraries
AssigneeTwist Bioscience Corporation
InventorsWilliam Banyai, Bill James Peck, Andres Fernandez, Siyuan Chen, Pierre Indermuhle
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Abstract
De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
Claims19
A method of de-novo synthesis of n-mer oligonucleotides on a substrate, comprising:
- a. providing a substrate with resolved loci in the form of microstructures that are functionalized with a chemical moiety suitable for nucleotide coupling;
- and
- b. coupling at least two nucleotide building blocks to a plurality of growing individual oligonucleotide chains, thereby synthesizing a plurality of oligonucleotides that are n bases long,
- wherein each oligonucleotide chain of the plurality of growing individual oligonucleotide chains resides on one resolved locus of the resolved loci,
- wherein the resolved loci comprise a pitch of no more than 0.1 mm, and
- wherein n is at least 50.
Dependencies are inferred from the claim text.