Closed loop, bedside cell purification systems and methods

A method for purifying a target cell from a patient blood sample in a closed-loop system, comprising;

• connecting a parenteral inlet adapted to parenterally receive blood from the patient;
• permitting the blood to pass through the parenteral inlet to an apheresis or leukopheresis module to provide a sample containing a target cell;
• permitting the sample containing the target cell to pass through the parenteral inlet to a cell purification module;
• introducing a targeting reagent comprising a substrate bound to a targeting moiety to the blood from the patient, or a fraction thereof, before or after the blood from the patient enters the cell purification module;
• permitting the targeting reagent to bind the target cell in the blood of the patient, or fraction thereof, to create a target cell/reagent complex in the cell purification module;
• separating the target cell/reagent complex from the blood from the patient, or fraction thereof;
• separating the target cell from the targeting reagent to create a separated target cell;
• and
• returning the separated target cell to the patient via a parenteral outlet adapted to parenterally administer the separated target cell to the patient,
• wherein each of the steps occur in the closed-loop system devoid of manual interventions such that each step occurs without removing the blood from the patient or any component of the blood from the patient from the close-loop system.