Decellularized tendon matrix methods and uses thereof
AssigneeThe Steadman Clinic and Steadman Philippon Research Institute
InventorsChelsea S. Bahney, Peter J. Millett, David Bernholt, Anna-Laura Nelson
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Abstract
Methods of making decellularized tendon matrix (DTM) and DTM hydrogels are provided. These compositions and hydrogels are useful for repairing tendon injuries and in some cases may be used by injection, arthroscopic procedures, or as adjuncts to traditional surgical repair.
Claims22
A decellularized tendon matrix (DTM) composition produced by a method comprising:
- decellularizing a minced native tendon tissue with a DNAse solution for about 0.5 to 2 hours thereby producing a decellularized tendon;
- contacting the decellularized tendon with an enzymatic solution comprising a matrix metalloproteinase (MMP) to produce a digested, decellularized tendon;
- lyophilizing the digested, decellularized tendon to produce a lyophilized tendon;
- reconstituting the lyophilized tendon to produce a DTM composition;
- wherein the DTM retains at least 50% of growth factors present in the native tendon;
- wherein the DTM is substantially free of TGFβ producing cells;
- and,
- wherein the method excludes decellularizing with anionic detergents comprising SDS and/or EDTA.
A tissue regeneration scaffold comprising a decellularized tendon matrix (DTM) material produced by a method comprising:
- decellularizing the native tendon thereby producing a decellularized tendon;
- contacting the decellularized tendon with an enzymatic solution comprising a matrix metalloproteinase (MMP) to produce a digested, decellularized tendon;
- lyophilizing the digested, decellularized tendon to produce a lyophilized tendon;
- and
- reconstituting the lyophilized tendon to produce the tissue regeneration scaffold, wherein the DTM retains at least 50% of growth factors present in the native tendon, and wherein the method excludes decellularizing with anionic detergents comprising SDS and/or EDTA.
A decellularized tendon matrix (DTM) composition produced by a method comprising:
- decellularizing a native tendon thereby producing a decellularized tendon;
- contacting the decellularized tendon with about 10 milliliters to about 50 milliliters of an enzymatic solution per 1 gram of tendon, or about 5 milliliters to about 10 milliliters of the enzymatic solution per 1 gram of tendon, wherein the enzymatic solution comprises a matrix metalloproteinase (MMP) to produce a digested, decellularized tendon;
- and
- lyophilizing the digested, decellularized tendon to produce a lyophilized tendon,
- wherein the method excludes decellularizing with anionic detergents comprising SDS and/or EDTA.
Dependencies are inferred from the claim text.