Combination grafts for tissue repair or regeneration applications

A method for producing a custom-designed dental graft for bony tissue repair of a dental extraction site which will become a dental implant site for an individual subject comprising:

• (a) before production of the dental graft, designing the dental graft with custom contours to allow for real-time formation of the dental graft to ensure proper fitment in the dental implant site of the individual subject and incorporation of matrices, growth factors and cell compositions appropriate for the individual subject in order to reduce dental implant failure;
• (b) preparing a growth factor composition comprising platelet-rich fibrin comprising a matrix of autologous fibrin in which platelet cytokines, growth factors, and cells comprising platelets are trapped from peripheral blood of a subject comprising bone morphogenetic protein-2;
• (c) preparing a cell culture composition comprising a suspended population of allogeneic mesenchymal stem cells in a culture medium comprising umbilical cord stem cells by expanding the suspended population of allogeneic mesenchymal stem cells in a culture medium comprising osteoconductive particles comprising a core containing tricalcium phosphate (TCP), hydroxyapatite (HA), bioactive glass, processed allograft particles or a combination thereof, wherein each osteoconductive particle is about 30 μm to about 40 μm, inclusive, in diameter, and wherein the cells of the cell culture composition attach on or to an osteoconductive surface of the particles;
• (d) mixing by centrifugation the growth factor composition of (b) with the cell culture composition of (c) to form a growth factor composition-cell culture composition mixture;
• (e) combining the growth factor composition-cell culture composition mixture with a three-dimensional carrier matrix comprising osteoconductive demineralized bone particles about 0.25 mm to about 1 mm in diameter, inclusive, wherein the carrier matrix is supersaturated by the population of cells of the cell culture composition, wherein the cells of the cell culture composition attach on or to an osteoconductive surface or within the pores of the osteoconductive demineralized bone particles;
• and wherein rate of degradation of the scaffold matches rate of tissue production by the population of cells;
• (f) forming the custom-designed dental graft into a shape appropriate for the anatomy and geometry of the dental implant site by milling and
• (g) inserting the dental graft into the dental implant site.