Multi-layer true spherical chitosan encapsulation of absolute (essential) oils for use in pesticidal activity

A method of encapsulating an active ingredient selected from the group consisting of a plant essential oil, a plant extract, an organic absolute botanical, and an organic essential oil, the method comprising:

• adding a first composition including at least a first active ingredient to a centrifuge;
• adding a solution containing chitosan into the centrifuge;
• centrifuging the chitosan-active ingredient mixture to create nanoparticle spheres of a first diameter;
• collecting the nanoparticle spheres of the first diameter;
• adding the nanoparticle spheres, a second composition including at least a second active ingredient, and a solution containing chitosan into a centrifuge;
• centrifuging the chitosan-active ingredient mixture to create nanoparticle spheres of a second diameter larger than the first diameter;
• and
• collecting the nanoparticle spheres of a second diameter, wherein
• the centrifuge comprises a nozzle having an internal portion with a diameter of 0.2-0.6 mm and an external portion with a diameter of 0.7-1.0 mm,
• the centrifuge operates at 4000-8000 rps, and
• the resulting nanoparticle spheres form a formulation effective for protecting plants from pests.

A method of chitosan encapsulation a pesticide active ingredient selected from the group consisting of a plant essential oil, a plant extract, an organic absolute botanical, and an organic essential oil, the method comprising:

• adding a first composition including at least a first active ingredient to a centrifuge;
• adding a solution containing chitosan into the centrifuge;
• centrifuging the chitosan and the first active ingredient mixture to create a first set of nanoparticle spheres comprising a core formed from the first composition and a first layer containing chitosan;
• collecting the first set of nanoparticle spheres;
• adding the collected first set of nanoparticle spheres, a second composition including at least a second active ingredient, and a solution containing chitosan into a centrifuge;
• centrifuging the mixture to create a second set of nanoparticle spheres comprising the core formed from the first composition, the first layer containing chitosan, a second layer formed from the second composition, and a third layer containing chitosan;
• wherein the centrifuge comprises a nozzle having an internal portion with a diameter of 0.2-0.6 mm and an external portion with a diameter of 0.7-1.0 mm, and
• collecting the second set of nanoparticle spheres, wherein the centrifuge operates at 4000-8000 rps, and the resulting nanoparticle spheres form a formulation effective for protecting plants from pests.