AI enabled selective raman spectrometry using microscopy for diagnosis and analysis of biological samples

A microscope assembly comprising:

• a first light source configured to emit illuminating radiations onto a sample slide;
• a focusing lens assembly positioned between the first light source and the sample slide that is configured to focus illumination radiations emitted by the first light source onto a sample positioned on the sample slide;
• a motorized stage configured to move the sample slide along one or both of an x axis and a y-axis within a plane;
• a plurality of objectives mounted on a motorized objective changer, wherein:
• each objective within the plurality of objectives comprises an optical assembly, and each of the plurality of objectives has optical properties that are different from other objectives within the plurality of objectives;
• and
• the motorized objective changer comprises a switching assembly configured to selectively interpose one of the plurality of objectives between the sample slide and an image sensor at an optical path along which illuminating radiations that have been reflected or scattered off the sample are directed;
• a first imaging apparatus having a first optical configuration;
• a second imaging apparatus having a second optical configuration;
• a beam splitter positioned at the optical path along which illuminating radiations that have been reflected or scattered off the sample, are directed, wherein the beam splitter is configured to selectively direct illuminating radiations that have been scattered or reflected off the sample to the first imaging apparatus or the second imaging apparatus;
• a processor configured for enabling raman spectroscopy image data from one or both of the first imaging apparatus and the second imaging apparatus to be transmitted to one or more processor implemented neural networks for analysis of raman spectroscopy image data;
• a second light source comprising one of a laser and LED light source, configured to emit light for excitation of the sample;
• a dichroic mirror assembly configured and positioned to direct light emitted from the second light source onto the sample;
• and
• a field diaphragm interposed between the second light source and the sample for controlling an amount of light projected from the second light source onto the sample.

A method of slide sample analysis, comprising:

• positioning a sample slide on a motorized stage;
• acquiring at a first image sensor, a plurality of images of the slide, each image including an image corresponding to a different portion of a sample disposed on the sample slide;
• storing each of the plurality of images in a memory, wherein each image is stored along with position coordinates defining a position of the portion of the sample that has been imaged therewithin;
• identifying within the combined image, one or more regions of interest of the sample;
• obtaining raman spectroscopy based image data corresponding to the identified one or more regions of interest of the sample;
• storing obtained raman spectroscopy data corresponding to each region of interest in the memory, wherein each instance of raman spectroscopy data is stored along with position coordinates defining a position of the region of interest within the sample to which the raman spectroscopy data corresponds;
• and
• determining based on the raman spectroscopy data whether the sample includes one or more states of interest.