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US 12,566,186 B2Granted
Assays for detecting the presence or amount of an anti-drug antibody
AssigneeGenzyme Corporation
InventorsRyan Grabert, Susan Richards, Valerie Theobald, Yuanxin Xu, Jad Zoghbi
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Abstract
Methods and kits for detecting antibodies (e.g., anti-drug antibodies). Such methods and kits permit the detection of, for example, anti-drug antibodies in human body fluids, such as blood, plasma and serum.
Claims19
A method for reducing interference in a drug assay due to the presence of an anti-drug antibody (ADA) in a sample, the method comprising:
- contacting the sample with an excess amount of an ADA to saturate free drug and form drug/ADA complexes;
- contacting the drug/ADA complexes with polyethylene glycol (PEG), to thereby form a precipitate comprising drug/ADA complexes;
- contacting the precipitate with a basic solution to dissociate the drug/ADA complexes;
- immobilizing the dissociated drug on a substrate;
- and
- performing the drug assay, to thereby reduce interference from the ADA.
3 more elements
Dependencies are inferred from the claim text.