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US 12,566,176 B2Granted
Flow cytometry system and methods for the diagnosis of infectious disease
AssigneeFlowMetric Life Sciences, Inc.
InventorsRenold Julius Capocasale, Julie Ann Bick
Life of this patent
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Abstract
Flow cytometry is used for diagnosis of infectious diseases by an analysis of cell mediated immune responses to specific infective agent antigens. Apparatus and methods of advanced flow cytometry are utilized to detect cell mediated immune responses to the presence of specific antigens from infective agents, such as bacteria, protozoa, viruses, helminth, prions. In some embodiments, methods as provided herein can be utilized in vitro to diagnose multiple infections within individuals.
Claims5
A kit for determining a presence of a pathogen in a subject, the kit comprising:
- one or more antigens of the pathogen;
- and
- a plurality of test tubes, each of the plurality of test tubes comprising an interior wall and having the one or more antigens of the pathogen coated on the interior wall,
- wherein when a biological sample obtained from the subject is exposed to the one or more antigens of the pathogen for a period of time sufficient to activate responding immune cells if the pathogen is present in the sample, the one or more antigens activate the responding immune cells to the pathogen in the sample;
- a first container comprising a first reagent, the first reagent comprising a first detectably-labeled antibody configured to bind to at least one activation marker of the responding immune cells in the sample;
- and
- a second container comprising a second reagent, the second reagent comprising a second detectably-labeled antibody configured to bind to one or more cytokines and/or chemokines secreted from the responding immune cells in the sample,
- wherein the labels of the first detectably-labeled antibody and the second detectably-labeled antibody are different.
5 more elements
Dependencies are inferred from the claim text.