Hemolytic reagent, reagent kit, and method for classifying white blood cells
AssigneeSysmex Corporation
InventorsYuki Shida, Takanori Komaki, Daisuke Yokoyama, Masaki Abe
Life of this patent
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Abstract
Disclosed is a hemolytic reagent comprising a nonionic surfactant represented by formula (I) below: R1—R2—(CH2CH2O)n—H (I) where R1 represents an alkyl group, an alkenyl group, or an alkynyl group having 8 or more and 25 or less carbon atoms, R2 represents an oxygen atom, (COO) or a group represented by formula (II) below: where n is 23 or larger and 25 or smaller, or 30, with n of 23 or larger and 25 or smaller, a concentration of the nonionic surfactant is 1700 ppm or higher and 2300 ppm or lower, and with n of 30, the concentration of the nonionic surfactant is 1900 ppm or higher and 2300 ppm or lower.
Claims16
A hemolytic reagent comprising a nonionic surfactant represented by formula (I) below:
- R1—R2—(CH2CH2O)n—H (I)
- where R1 represents an alkyl group, an alkenyl group, or an alkynyl group having 8 or more and 25 or less carbon atoms,
- R2 represents an oxygen atom, (COO) or a group represented by formula (II) below:
- where n is 23 or larger and 25 or smaller, or n is 30,
- wherein when n is 23 or larger and 25 or smaller, a concentration of the nonionic surfactant is 1700 ppm or higher and 2300 ppm or lower, and
- when n is 30, the concentration of the nonionic surfactant is 1900 ppm or higher and 2300 ppm or lower.
A method for classifying white blood cells, comprising:
- detecting optical information by irradiating a light on a measurement sample prepared by mixing a hemolytic reagent, a staining reagent for fluorescently staining nucleic acid, and a sample that contains white blood cells;
- and
- classifying the white blood cells on the basis of the optical information,
- the hemolytic reagent containing a nonionic surfactant represented by formula (I) below:
- R1—R2—(CH2CH2O)n—H Formula (I)
- where R1 represents an alkyl group, an alkenyl group, or an alkynyl group having 8 or more and 25 or less carbon atoms,
- R2 represents an oxygen atom, (COO) or a group represented by formula (II) below:
- where n is 23 or larger and 25 or smaller, or 30,
- wherein when n is 23 or larger and 25 or smaller, a concentration of the nonionic surfactant in the measurement sample is 1650 ppm or higher and 2250 ppm or lower, and
- when n is 30, a concentration of the nonionic surfactant in the measurement sample is 1850 ppm or higher and 2250 ppm or lower.
Dependencies are inferred from the claim text.