Method analysis employing measurement based on polarization anisotropy

An analysis apparatus for measuring a value (R) for polarization anisotropy through use of a luminescent reagent that reacts with a target substance,

• configured to measure at least any one of the presence or absence of the target substance and a concentration of the target substance,
• the analysis apparatus comprising:
• a reaction unit configured to mix a sample containing the target substance with the luminescent reagent, and to subject the mixture to a reaction to obtain a reaction liquid;
• a dilution unit configured to dilute the reaction liquid to obtain a diluted liquid;
• a measurement unit configured to measure the R of the diluted liquid;
• and
• a control unit,
• wherein the luminescent reagent includes including in luminescent particles, and
• wherein the R is defined to be “r” in equation (1);
• r
• =
• I
• VV
• -
• G
• *
• I
• VH
• I
• VV
• +
• 2
• *
• G
• *
• I
• VH
• (
• 1
• )
• G
• =
• I
• HV
• I
• HH
• ,
• wherein:
• IVV represents a luminescence intensity of a luminescence component having a vibration direction parallel to that of a first polarized light beam at a time of excitation by the first polarized light beam;
• IVH represents a luminescence intensity of a luminescence component having a vibration direction orthogonal to that of the first polarized light beam at the time of excitation by the first polarized light beam;
• IHV represents a luminescence intensity of a luminescence component having a vibration direction orthogonal to that of a second polarized light beam having a vibration direction orthogonal to that of the first polarized light beam at a time of excitation by the second polarized light beam;
• IHH represents a luminescence intensity of a luminescence component having a vibration direction parallel to that of the second polarized light beam having a vibration direction orthogonal to that of the first polarized light beam at the time of excitation by the second polarized light beam;
• and
• G represents a correction value.