Methods of using modular assay support devices
Assignee10x Genomics, Inc.
InventorsDavid Maurice Cox, Layla Katiraee
Life of this patent
Estimating the term…
Abstract
A substrate holder includes a base configured to receive a substrate; a cover configured to mateably engage with the base, the cover defining an opening formed by inner sidewalls; and a removable insert defining a surface, the removable insert being configured to be received within the opening of the cover. The removable insert includes a gasket; a projection coupled to the gasket; and at least two insert tabs extending from opposite sides of the removable insert, each insert tab being configured to engage with at least one of the inner sidewalls forming the opening of the cover.
Claims30
A method comprising:
- mounting a substrate on a base of a substrate holder, the substrate comprising a sample region supporting at least one sample, the base comprising a first base tab extending laterally outward from and along a first side of a perimeter of the base and a second base tab extending laterally outward from and along a second side of the perimeter of the base, the second side of the perimeter of the base arranged opposite the first side of the perimeter of the base;
- releasably locking a cover of the substrate holder to the base, the cover comprising a first cover tab extending laterally outward from and along a first side of a perimeter of the cover and a second cover tab extending laterally outward from and along a second side of the perimeter of the cover, the second side of the perimeter of the cover being arranged opposite the first side of the perimeter of the cover, wherein releasably locking the cover to the base comprises releasably locking the first cover tab to the first base tab and the second cover tab to the second base tab thereby forming a well around the sample region;
- and
- after releasably locking the cover to the base, contacting the at least one sample with a reagent by adding the reagent to the well through an opening arranged within the perimeter of the cover.
A method comprising:
- mounting a substrate on a base of a substrate holder, the substrate comprising a sample region supporting at least one sample, and the base comprising:
- a bottom portion extending from a first end to a second end;
- a first side wall extending from the first end of the bottom portion;
- a second side wall extending from the second end of the bottom portion;
- a first base tab extending laterally outward from and along at least a portion of an outer surface of the first side wall;
- and
- a second base tab extending laterally outward from and along at least a portion of an outer surface of the second side wall, the second side wall arranged opposite the first side wall;
- releasably locking a cover of the substrate holder to the base, the cover comprising:
- a main body extending from a first end to a second end, the second end being arranged opposite the first end;
- a first cover tab extending laterally outward from and along at least a portion of the first end;
- and
- a second cover tab extending laterally outward from and along at least a portion of the second end, wherein releasably locking the cover to the base comprises releasably locking the first cover tab to the first base tab and the second cover tab to the second base tab thereby forming a well around the sample region;
- and
- contacting the at least one sample with a reagent by adding the reagent to the well through an opening formed in the cover.
Dependencies are inferred from the claim text.