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US 12,565,672 B2Granted
Sampling method and kit for histamine measurement
AssigneeKikkoman Corporation
InventorYuko Ichiyanagi
Life of this patent
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Abstract
An object of the present invention is to provide a convenient sampling method and kit for histamine measurement. The present invention provides a method and a kit which involve non-invasively sampling a specimen, and detecting histamine from the obtained sample.
Claims4
A method of measuring only histamine for the examination of histamine food poisoning or for the prevention of histamine food poisoning, comprising the steps of:
- (i) non-invasively sampling a specimen to obtain a sample for measurement from the specimen;
- and
- (ii) subjecting the sample obtained by the sampling in step (i) to a histamine measurement,
- wherein the histamine measurement is measured by colorimetry using an enzyme, wherein the enzyme consists of a histamine dehydrogenase, and a mediator, or by immunochromatography using an anti-histamine antibody,
- wherein the sample for measurement is collected without damaging the specimen for the sampling,
- wherein the specimen is a fish or a chicken, and wherein
- when the specimen is a fish, the non-invasive sampling is performed from the mouth, a gill slit, anus, or peritoneal cavity, or
- when the specimen is a chicken, the non-invasive sampling is performed from the mouth, anus, or on a surface of a cut of chicken meat that is not minced.
5 more elements
A method of measuring histamine for the examination of histamine food poisoning or for the prevention of histamine food poisoning, the method comprising the steps of:
- (i) non-invasively sampling a specimen to obtain a sample for measurement from the specimen;
- and
- (ii) subjecting the sample obtained by the sampling in step (i) to a histamine measurement,
- wherein the histamine measurement is measured by colorimetry using a histamine dehydrogenase and a mediator or by immunochromatography using an anti-histamine antibody, and further using an error reaction suppressor,
- wherein the sample for measurement is collected without damaging the specimen for the sampling,
- wherein the specimen is a fish or a chicken, and wherein
- when the specimen is a fish, the non-invasive sampling is performed from the mouth, a gill slit, anus, peritoneal cavity, on a surface of the fish body, or on a surface of a cut of fish meat that is not minced, or
- when the specimen is a chicken, the non-invasive sampling is performed from the mouth, anus, or on a surface of a cut of chicken meat that is not minced,
- wherein the error reaction suppressor is at least one selected from the group consisting of:
- (1) boric acid or a salt thereof;
- (2) a boronic acid represented by the following formula (I) or (II):
- wherein R1, R2, R3, R4 and R5 are each independently selected from the group consisting of H, a boronyl group, a halogen group, a hydroxy group, a carboxy group, a nitro group, an amino group, a sulfo group, a thiol group, a tert-butoxycarbonylamino group, a substituted or unsubstituted C1 to C10 alkyl group, a substituted or unsubstituted alkenyl group, and a substituted or unsubstituted alkynyl group, and
- R6 is selected from the group consisting of a substituted or unsubstituted C1 to C10 alkyl group, a substituted or unsubstituted alkenyl group, and a substituted or unsubstituted alkynyl group, wherein the substituted C1 to C10 alkyl group, the substituted alkenyl group or the substituted alkenyl group is substituted with at least one substituent selected from the group consisting of a boronyl group, a halogen group, a hydroxy group, a carboxy group, a nitro group, an amino group, a sulfo group and a thiol group,
- or a salt thereof;
- (3) alkyl sulfate;
- and
- (4) a buffer selected from the group consisting of BES, MOPS, TES, HEPES, EPPS, TAPS, CHES, CAPS, TAPSO, POPSO, HEPPSO, ACES, Bis-Tris, MES, MOPSO, PIPES, Tris (tris(hydroxymethyl)aminomethane), and a carbonate buffer.
14 more elements
Dependencies are inferred from the claim text.