Genome-edited birds
AssigneeThe State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organization (ARO) (Volcani Center)
InventorsYuval Cinnamon, Enbal Ben-Tal Cohen
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Abstract
The present disclosure provides exogenous polynucleotide cassettes for generating chimeric bird cells and chimeric birds. The polynucleotide cassettes can be used to produce conditionally-lethal phenotype in male bird embryos. In one embodiment, the present disclosure provides methods for destroying male chick embryos in-ovo.
Claims30
A DNA editing agent, comprising a polynucleotide cassette having a formula 5′-LHA (left homology arm)-OIE (optogenetic-inducible element)-LIE (lethality-inducing element)-RHA (right homology arm)-3′ or a formula 5′-LHA-LIE-OIE-RHA-3′, wherein
- (i) the LHA comprises a first nucleotide sequence that is substantially homologous to a first corresponding nucleotide sequence on chromosome Z of a bird;
- (ii) the OIE comprises a first promoter functionally linked to a second nucleotide sequence encoding an inducer-activated site-specific recombinase enzyme;
- (iii) the LIE comprises a third nucleotide sequence encoding a lethality-promoting protein, which is operatively linked to the activity of the inducer-activated site-specific recombinase enzyme;
- and
- (iv) the RHA comprises a fourth nucleotide sequence that is substantially homologous to a second corresponding nucleotide sequence on chromosome Z of a bird;
- wherein the first corresponding nucleotide sequence or the second corresponding nucleotide sequence is located in an openly transcribed region on chromosome Z.
Dependencies are inferred from the claim text.