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US 12,565,663 B2Granted
Matrix attachment regions and uses in promoting gene expression
AssigneeCelltheon Corporation
InventorNikhil Goel
Life of this patent
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Abstract
The present disclosure relates to new identified matrix attachment regions (MAR) and chimeric sequences. Also disclosed are nucleotide constructs containing a MAR at a suitable location relative to a protein-coding sequence for optimal expression of the protein.
Claims2
An engineered recombinant expression cassette on the same polynucleotide consisting of (i) a heterologous promoter operably linked to (ii) a coding sequence encoding a transgene to initiate the transcription of the coding sequence, and (iii) a matrix attachment region (MAR) nucleic acid sequence comprising SEQ ID NO:
- 1 or SEQ ID NO:4, wherein the MAR is capable of attaching to a mammalian nuclear matrix and enhancing gene expression in cells;
- wherein the heterologous promoter is not naturally associated with the MAR sequence in its native genomic context.
Dependencies are inferred from the claim text.