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US 12,565,659 B2Granted
Recombinant lac polynucleotides and uses thereof to increase production of C-lignin in plants
AssigneeUniversity of North Texas
InventorsRichard A. Dixon, Fang Chen, Chunliu Zhuo
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Abstract
The disclosure provides recombinant LAC polynucleotides encoding a laccase (LAC) polypeptide capable of polymerizing caffeyl alcohol, vectors and cells including the recombinant LAC polynucleotide. The disclosure also provides transgenic plants including cells having the recombinant LAC polynucleotide of the present disclosure and methods of increasing production of C-lignin in a plant.
Claims15
A recombinant polynucleotide comprising:
- a laccase (LAC) polynucleotide encoding a LAC polypeptide capable of polymerizing caffeyl alcohol, wherein the LAC polypeptide is a conservative variant of the LAC polypeptide of SEQ ID NO:3 having a sequence that is at least 95% identical to SEQ ID NO:3 with only conservative substitutions compared to SEQ ID NO:3, and having a glutamine residue in position 289 (Q289);
- and at least one heterologous polynucleotide sequence operatively linked to the LAC polynucleotide, wherein the at least one heterologous polynucleotide sequence comprises a regulatory polynucleotide sequence, promoter sequence, a selectable marker polynucleotide, or a combination thereof.
Dependencies are inferred from the claim text.