Enhanced platforms for unnatural amino acid incorporation in mammalian cells
AssigneeThe Trustees of Boston College
InventorsAbhishek Chatterjee, Rachel E. Kelemen, Delilah Jewel
Life of this patent
Estimating the term…
Abstract
The present invention involves the ability to 1) use a virus assisted directed evolution platform to significantly improve the activity of engineered nonsense-suppressor tRNAs in mammalian cells, 2) provide mutants of archaeal pyrrolysyl and E. coli leucyl tRNAs that show remarkably improved Uaa incorporation efficiency in mammalian cells, and 3) use these tRNAs to express recombinant proteins in mammalian cells incorporating Uaas at significantly improved yields.
Claims23
A composition comprising a variant archaeal or variant bacterial suppressor tRNA, wherein the variant tRNA has increased activity relative to its wild-type counterpart tRNA to incorporate an unnatural amino acid into a protein produced in mammalian cells, wherein the activity of the variant tRNA is increased over the wild-type tRNA by about 10 to 80-fold.
Dependencies are inferred from the claim text.