Engineered terminal deoxynucleotidyl transferase variants
AssigneeCodexis, Inc.
InventorsCharlene Ching, Stephanie Marie Forget, Anders Matthew Knight, Mikayla Jianghongxia Krawczyk, Niusha Mahmoodi, Melissa Ann Mayo, Mathew G. Miller, Jonathan Vroom
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Abstract
The present invention provides engineered terminal deoxynucleotidyl transferase (TdT) polypeptides useful in template-independent polynucleotide synthesis using a nucleoside triphosphate-3′-O-removable blocking group (NTP-3′-O-RBG), as well as compositions, methods of utilizing these engineered polypeptides, and polynucleotides encoding the engineered terminal deoxynucleotidyl transferases.
Claims42
An engineered terminal deoxynucleotidyl transferase comprising a a polypeptide sequence having at least 90% sequence identity to SEQ ID NO:
- 5296, wherein the engineered terminal deoxynucleotidyl transferase comprises C68R in its polypeptide sequence, and wherein said engineered terminal deoxynucleotidyl transferase has terminal deoxynucleotidyl transferase activity and wherein the amino acid positions of the polypeptide sequence are numbered with reference to SEQ ID NO:
- 5296.
Dependencies are inferred from the claim text.