Method of differentiating pluripotent or epiblast cells into immature oocytes
AssigneeDioseve Inc.
InventorsNobuhiko Hamazaki, Katsuhiko Hayashi
Life of this patent
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Abstract
A method for inducing immature oocytes includes introducing four genes consisting of FIGLA, NOBOX, LHX8 and TBPL2, or transcripts or expressed proteins thereof, into at least one type of cell selected from the group consisting of pluripotent stem cells, epiblast-like cells and primordial germ cells. A method for producing mature oocytes includes introducing four genes consisting of FIGLA, NOBOX, LHX8 and TBPL2, or transcripts or expressed proteins thereof, into at least one type of cell selected from the group consisting of pluripotent stem cells, epiblast-like cells and primordial germ cells, and co-culturing the cell obtained after the introduction and ovarian somatic cells.
Claims9
A method for making an immature oocyte, the method comprising:
- (a) introducing nucleic acid sequences encoding folliculogenesis-specific basic helix-loop-helix (FIGLA), newborn oogenesis homeobox (NOBOX), LIM homeobox 8 (LHX8), and TATA-box binding protein like 2 (TBPL2) into an isolated mammalian pluripotent or epiblast cell, and
- (b) culturing the cell obtained in step (a) in a pluripotent medium such that an immature oocyte is obtained.
A method for making a mature oocyte, the method comprising:
- (a) introducing nucleic acid sequences encoding folliculogenesis-specific basic helix-loop-helix (FIGLA), newborn oogenesis homeobox (NOBOX), LIM homeobox 8 (LHX8), and TATA-box binding protein like 2 (TBPL2) into an isolated mammalian pluripotent cell,
- (b) culturing the cell obtained in step (a) in a pluripotent medium such that an immature oocyte is obtained, and
- (c) culturing the immature oocyte obtained in step (b) in a medium comprising follicular stimulating hormone, epidermal growth factor (EGF), human chorionic gonadotropin (hGC), and serum such that a mature oocyte is obtained.
Dependencies are inferred from the claim text.