FCRN/antigen-binding molecules and methods of use
Assigneeargenx BV
InventorsVladimir Bobkov, Karen Silence, Jolien Van Santbergen, René Bigirimana, Judith Baumeister, Johannes de Haard, Christophe Blanchetot
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Abstract
Provided herein are binding molecules comprising a human neonatal Fc receptor (FcRn) binding molecule and at least one antigen-binding domain linked to the FcRn binding molecule. Polynucleotides, vectors, host cells, and methods of production are also provided herein. Methods of treating an antibody-mediated disorder with an FcRn/antigen-binding molecule are further provided.
Claims11
A heterodimeric protein comprising a human IgG1 Fc region and a single VHH that specifically binds to human serum albumin (HSA), wherein:
- (a) the human IgG1 Fc region comprises (i) a first human IgG1 Fc domain comprising amino acids Y, T, E, W, K, and Y at EU positions 252, 254, 256, 366, 433, and 434, respectively, and (ii) a second human IgG1 Fc domain comprising amino acids Y, T, E, S, A, V, K, and Y at EU positions 252, 254, 256, 366, 368, 407, 433, and 434, respectively;
- (b) the VHH comprises the amino acid sequence of SEQ ID NO:
- 42;
- and
- (c) the VHH is fused to the C-terminal end of the first Fc domain or the second Fc domain via a (GGGGS)n peptide linker, wherein n is 1 to 8.
A heterodimeric protein comprising:
- (a) a first polypeptide comprising from N-terminus to C-terminus:
- (i) a first human IgG1 Fc domain comprising amino acids Y, T, E, S, A, V, K, and Y at EU positions 252, 254, 256, 366, 368, 407, 433, and 434, respectively, (ii) a (GGGGS)n peptide linker, wherein n is 1 to 8, and (iii) a VHH comprising the amino acid sequence of SEQ ID NO:
- 42;
- and
- (b) a second polypeptide comprising a second human IgG1 Fc domain comprising amino acids Y, T, E, W, K, and Y at EU positions 252, 254, 256, 366, 433, and 434, respectively, and wherein the second polypeptide does not comprise an antigen-binding domain.
A heterodimeric protein comprising:
- (a) a first polypeptide comprising from N-terminus to C-terminus:
- (i) a first human IgG1 Fc domain comprising amino acids Y, T, E, W, K, and Y at EU positions 252, 254, 256, 366, 433, and 434, respectively, (ii) a (GGGGS)n peptide linker, wherein n is 1 to 8, and (iii) a VHH comprising the amino acid sequence of SEQ ID NO:
- 42;
- and
- (b) a second polypeptide comprising a second human IgG1 Fc domain comprising amino acids Y, T, E, S, A, V, K, and Y at EU positions 252, 254, 256, 366, 368, 407, 433, and 434, respectively, and wherein the second polypeptide does not comprise an antigen-binding domain.
Dependencies are inferred from the claim text.