Back to results
US 12,565,519 B2Granted
Recombinant strains and medium formulation for enhancing secretion titer using a type III secretion system
AssigneeNorthwestern University
InventorsDanielle Tullman Ercek, Lisa A. Burdette, Han Teng Wong
Life of this patent
Estimating the term…
Abstract
The present disclosure provides a recombinant Salmonella strain having a Type III secretion system (T3SS) comprising mutation which enhance protein expression and production. Additionally, methods and kits for using the recombinant Salmonella strain for producing a protein of interest are provided. Additionally, an optimized medium that increases protein expression in a Salmonella strain having a Type III secretion system (T3SS) is provided.
Claims22
A recombinant Salmonella strain having a Type III secretion system (T3SS) comprising
- (a) an insertion of a protein coding sequence between the hill coding sequence and the hilD 3′UTR suitable to increase hilD expression in the strain;
- or
- (b) an insertion of a protein coding sequence between the hill coding sequence and the hilD 3′UTR suitable to increase hilD expression in the strain;
- and a deletion of hilE;
- wherein the protein coding sequence encodes a B-barrel protein.
2 more elements
Dependencies are inferred from the claim text.