Thermoformed, injection molded, and/or overmolded microfluidic structures and techniques for making the same

A method comprising:

• (a) forming a laminated microfluidic structure having a first major surface and a second major surface offset from the first major surface by a first distance along an axis perpendicular to the first major surface, wherein the laminated microfluidic structure includes:
• at least two layers, wherein each layer is made of a polymeric material,
• at least one channel that extends in one or more directions parallel to the first major surface and is located within the laminated microfluidic structure, and
• one or more sipper ports that are fluidically connected with one or more features in the laminated microfluidic structure and outside of a first region by the at least one channel;
• (b) placing the laminated microfluidic structure in a thermoforming apparatus;
• and
• (c) thermoforming the first region of the laminated microfluidic structure around the one or more sipper ports to cause the first region to distend away from a reference plane defined by undistended portions of the first major surface to form a well with the one or more sipper ports located within the distended first region.