Nucleic acid mazzocchio and methods of making and use thereof

A nucleic acid nanostructure having a continuous torus-like structure with a closed three-dimensional cavity, the nanostructure comprising a plurality of subunits,

• wherein each of the subunits comprises a core domain and a connecting domain,
• wherein the core domain defines a polygon having a plurality of edges that enclose an open area,
• wherein the edges and open area define a plane of the subunit,
• wherein the connecting domain comprises one or more linker nucleic acid strands,
• wherein a first edge of each subunit is coplanar with the first edges of the other subunits,
• wherein each of the subunits is between and connected to two other of the subunits with the planes of the connected subunits facing each other thereby forming a stack of subunits,
• wherein the planes of the connected subunits are substantially perpendicular to the plane of the first edges of the subunits and are tilted relative to the subunits connected to a given subunit in the direction horizontal to the plane of the first edges of the subunits,
• wherein the tilt between the planes of connected subunits produces a curve in the stack of subunits whereby the connected subunits form the continuous torus-like structure with a closed three-dimensional cavity defined by the open areas of the stacked subunits,
• wherein the core domain of each subunit comprises a scaffold nucleic acid strand and staple nucleic acid strands,
• wherein the connection between the subunits is made by the linker nucleic acid strands, wherein each linker comprises a length,
• wherein the length is configured to create the tilt between the planes of connected subunits and thereby produce the continuous torus-like structure,
• wherein each subunit comprises 6-20 single-stranded nucleic acid strands, and
• wherein the subunits and linkers in the nanostructure form an extended supramolecular corkscrew.