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US 12,564,607 B2Granted
Cell population comprising mesenchymal cells, pharmaceutical composition comprising the same, and method for producing the same
AssigneeKaneka Corporation
InventorsNobuyoshi Umeda, Kohei Ogura
Life of this patent
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Abstract
This invention provides a cell population comprising mesenchymal cells capable of forming a cell sheet that can be spontaneously detached from a substrate. In such cell population comprising mesenchymal cells, the proportion of cells positive for CD324 is 70% or more and the proportion of mesenchymal cells positive for CD90 is 90% or more.
Claims2
A method for producing a cell population comprising mesenchymal cells comprising:
- a step of culturing a cell population comprising mesenchymal cells derived from a fetal appendage to obtain a cultured cell population;
- subculturing said cultured cell population in a culture medium which contains an effective amount of human platelet lysate which does not contain fetal bovine serum to produce a subcultured cell population;
- and
- a step of selecting a subcultured cell population having the properties (a), (b), and (c) below from among the cell populations comprising mesenchymal cells:
- (a) a cell population in which the proportion of cells positive for CD324 is 80% or more;
- (b) a cell population in which the proportion of mesenchymal cells positive for CD90 is 90% or more;
- (c) a cell population in which the proportion of mesenchymal cells positive for CD326 is 5% or less;
- and
- wherein the medium in said subculturing contains 3% to 20% by weight of human platelet lysate.
6 more elements
Dependencies are inferred from the claim text.