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US 12,564,179 B2Granted
Methods of producing granulomas and animal models of sarcoidosis
AssigneesThe United States Government as represented by the Department of Veterans Affairs, University of Miami
InventorsMehdi Mirsaeidi, Chongxu Zhang
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Abstract
The disclosure relates to a sarcoidosis animal model and methods of inducing sarcoidosis in an animal. The disclosure also relates to methods of producing an in vitro granuloma, and methods of using the sarcoidosis animal model. Disclosed herein are animals comprising one or more granulomas, wherein the one or more granulomas comprise Mycobacterium abscessus cell wall microparticles.
Claims8
A method of producing a Mycobacterium abscessus cell wall microparticle, the method comprising:
- (a) obtaining cells from a rough colony of one or more strains of Mycobacterium abscessus;
- (b) lysing the cells in a lysis buffer, wherein the lysis buffer comprises a protease inhibitor and a detergent;
- (c) centrifuging the cells in (b) so as to produce a pellet and a supernatant liquid;
- and collecting the supernatant;
- (d) centrifuging the supernatant so as to produce a second pellet and a second supernatant liquid;
- and collecting the second pellet;
- and
- (e) contacting the second pellet with a lysis buffer and heating to a temperature of at least 90° C., wherein the lysis buffer comprises a protease inhibitor and a detergent;
- thereby forming Mycobacterium abscessus cell wall microparticles.
6 more elements
Dependencies are inferred from the claim text.