Microscopic observation method and microscopic observation device

A microscopic observation method, configured to observe a specimen in a room formed between a bottom, a wall standing on the bottom and a cover disposed on the wall of a specimen carrier made of light-passable material, wherein steps of the microscopic observation method comprise:

• placing the specimen carrier at an observation point;
• obtaining a length of the bottom or the cover of the specimen carrier along a movement direction, a thickness of the wall of the specimen carrier along an observation direction of a microscope objective, an observation angle of the microscope objective, and a relative distance between a lateral surface of the wall of the specimen carrier and the microscope objective along the movement direction;
• and
• adjusting an incident angle of a light beam emitted from a dark-field illumination towards the specimen carrier according to a calculation result of the length, the thickness, the observation angle, and the relative distance for preventing the light beam emitting towards an observation area of the microscope objective;
• wherein an optical path of the light beam emitted from the dark-field illumination to the observation point is located on an opposite side of the bottom of the specimen carrier away from the microscope objective.