Back to results
US 12,560,614 B2Granted
Method for diagnosing and treating fibromyalgia
AssigneeCenter for Immunology Science
InventorBruce S. Gillis
Life of this patent
Estimating the term…
Abstract
The invention provides methods, kits and reagents for diagnosing fibromyalgia (FM) in an individual by determining whether the levels of one or more cytokines in the individual are altered, as compared to control levels. The altered level(s) or patterns of expression of the cytokines measured in the affected individual compared to the level from the control is predictive/indicative of FM in the individual.
Claims8
A method for detecting nucleic acid expression of cytokines in a peripheral blood mononuclear cell (PBMC) sample obtained from an individual previously diagnosed with fibromyalgia, comprising:
- (a) stimulating the PBMC sample with one or more mitogens;
- and (b) measuring the nucleic acid expression levels in the PBMC sample of at least four cytokines selected from the group consisting of interleukin 5 (IL5), interleukin 6 (IL6), interleukin 8 (IL8), interleukin10 (IL 10), interferon-γ (IFN-γ), monocyte chemotactic protein 1 (MCP-1), macrophage inflammatory protein-1α (MIP-1α) and macrophage inflammatory protein-1β (MIP-1β) at the nucleic acid level by quantitative RT-PCR, Northern Blot assay, array hybridization or sequencing, thereby detecting the nucleic acid expression of the at least four cytokines in the PBMC sample.
Dependencies are inferred from the claim text.