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US 12,560,608 B2Granted
Detection of molecular associations
AssigneeCalifornia Institute of Technology
InventorsMitchell Guttman, Mario R. Blanco, Ward Walkup, IV
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Abstract
In some embodiments, methods of detecting an association between a query protein and a target moiety are described. In some embodiments, compositions are described. In some embodiments, kits are described.
Claims18
A kit comprising:
- a. a vector comprising 1) an insertion site, 2) a tag coding sequence, and 3) a counterpart polypeptide coding sequence;
- wherein
- the insertion site is arranged to place a query protein coding sequence in frame with the tag coding sequence without any intervening stop codon,
- wherein
- upon the insertion of the query protein coding sequence in the insertion site the vector encodes a tagged query protein with the counterpart polypeptide sequence located at the N-terminal region of the query protein;
- b. a covalent polypeptide tag, wherein the covalent polypeptide tag and the counterpart polypeptide sequence are configured to specifically covalently bind to each other;
- c. a substrate configured to specifically bind covalently to the encoded tag, and
- d. a crosslinking agent.
5 more elements
A composition comprising:
- a vector comprising an insertion site, a tag coding sequence, and a counterpart polypeptide coding sequence, wherein
- the insertion site is arranged to place a query protein coding sequence in frame with the tag coding sequence without any intervening stop codon, wherein
- upon the insertion of the query protein coding sequence in the insertion site the vector encodes a tagged query protein with the counterpart polypeptide sequence located at the N-terminal region of the query protein;
- and a covalent polypeptide tag, wherein
- the covalent polypeptide tag and the counterpart polypeptide sequence are configured to specifically covalently bind to each other.
2 more elements
Dependencies are inferred from the claim text.