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US 12,560,607 B2Granted
Recombinant viruses, insect cells and their uses in viral detection and vaccination
AssigneeAcademia Sinica
InventorsYu-Chan Chao, Sung-Chan Wei, Huei-Ru Lo, Chih-Hsuan Tsai, Yu-Wen Lo, Wei-Ting Hsu, Chuan-Yu Liao
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Abstract
Provided herein are recombinant viruses and/or insect cells suitable for detecting the infection of a pathogen in a biological sample of a test subject. The information derived from the detection may also be used to render a diagnosis on whether the test subject is infected with the pathogen or not, so that proper course of treatment may be assigned to the subject. Also provided herein is a vaccine for the prophylaxis and/or treatment of infection caused by the pathogen.
Claims10
A recombinant baculovirus comprising:
- a promotor consisting of the promoter of the p10 gene and the promoter of the hr1-hsp70 gene;
- a first nucleic acid operably linked to the promoter, wherein the first nucleic acid encodes the S or N protein of a coronavirus to be expressed on the outer surface of the recombinant virus;
- a second nucleic acid disposed upstream to the first nucleic acid encoding a signal peptide selected from the group consisting of a honeybee melittin signal peptide (HM/HBM), a GP64 signal peptide, a cecropin B signal peptide, and a GRP78/Bip signal peptide, and
- a third nucleic acid encoding a transmembrane domain and a cytoplasmic tail domain of a baculovirus glycoprotein GP64 operably linked to the promotor.
1 more element
Dependencies are inferred from the claim text.