Compositions for determining vaccine potency
AssigneeBioMadison, Inc.
InventorWard C. Tucker
Life of this patent
Estimating the term…
Abstract
Compositions for determining the efficacy and/or potency of a vaccine preparation are described herein. Splenocytes from immunized animals are isolated and can be frozen. Upon thawing such cells are activated by exposure to a series of dilutions of a vaccine preparation being tested and a series of dilutions of a reference vaccine with known characteristics. Cells secreting immunogen-specific antibody and cells secreting nonspecific antibody are enumerated, as is the amount of immunogen-specific and nonspecific antibody produced. Comparison between the results from the vaccine preparations provides a measure of relative vaccine efficacy and/or potency.
Claims6
A cell culture for determining vaccine potency of a test vaccine preparation, comprising a cell preparation comprising splenocytes collected from a spleen of an animal immunized with a reference vaccine and prepared for storage, wherein the splenocytes are reactivated by exposure to serial dilutions of the reference vaccine following retrieval from storage.
Dependencies are inferred from the claim text.