Rapid analysis of live cells
AssigneesMassachusetts Institute of Technology, Dana-Farber Cancer Institute, Inc.
InventorsDavid Weinstock, Scott R. Manalis, Robert J. Kimmerling, Selim Olcum
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Abstract
A method of rapid functional analysis of cells is provided. A body fluid sample is introduced into a reservoir of a measurement instrument. A living cell is loaded directly from the body fluid sample into a channel of the measurement instrument in the absence of long-term cell culturing, cell passaging, and application of long-term drug pressure to cells. A functional biomarker of the living cells is measured while the living cell flows through the channel. The functional biomarker measured may be mass accumulation rate (MAR) or mass change. The measurement instrument may be a suspended microchannel resonator (SMR).
Claims16
A method of rapid functional analysis of cells comprising:
- obtaining a sample comprising a live cell from a patient by drawing the sample from a solid tumor by fine needle aspiration and disaggregating the sample to release the live cell;
- introducing the live cell into an input reservoir of a measurement instrument, wherein the measurement instrument is a suspended microchannel resonator (SMR);
- flowing the live cell through a channel of the measurement instrument;
- and
- measuring, within less than about 3 hours after the sample is drawn from the patient, a functional biomarker of the live cell while it flows through the channel, wherein the biomarker is mass or mass accumulation rate (MAR)
- wherein the method is performed without an intervening period for culturing the live cell.
A method of rapid functional analysis of cells comprising:
- obtaining a sample comprising a live cell from a patient by drawing the sample from a solid tumor by fine needle aspiration and disaggregating the sample to release the live cell;
- introducing the live cell into an input reservoir of a measurement instrument, wherein the measurement instrument is a suspended microchannel resonator (SMR);
- flowing the live cell through a channel of the measurement instrument;
- and
- measuring, within less than about 3 hours after the obtaining of the sample, a functional biomarker of the live cell while it flows through the channel, wherein the biomarker is mass or mass accumulation rate (MAR),
- wherein the method is performed without in vitro culture of the live cell, and
- wherein the measurement instrument comprises a dosing component that treats the live cell with a therapeutic.
Dependencies are inferred from the claim text.