Ultrabright fluorescent nanocomposite structures for enhanced fluorescent bioassays
AssigneeAuragent Bioscience, LLC
InventorsClare Kercher, Qisheng Jiang, Scott L. Crick
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Abstract
Described herein is a fluorescent nanocomposite. The fluorescent nanocomposite structure may include a plasmonic nanostructure comprising having at least one localized surface plasmon resonance wavelength (λLSPR), at least one spacer coating, at least one fluorescent agent having a maximum excitation wavelength (λEX), and at least one peptide-loaded major histocompatibility complex (MHC) molecule (pMHC). The fluorescent nanocomposite structure has a fluorescent intensity that is at least 500 times greater than a fluorescent intensity of the at least one fluorescent agent alone.
Claims8
A fluorescent nanocomposite structure comprising:
- a plasmonic nanostructure having at least one localized surface plasmon resonance wavelength (λLSPR);
- a spacer layer coating the plasmonic nanostructure;
- at least one fluorescent agent having a first maximum excitation wavelength (λEX1) and a first maximum emission wavelength (λEM1) conjugated on the spacer layer;
- and
- at least one fluorescent agent having a second maximum excitation wavelength (λEX2) and a second maximum emission wavelength (λEM2) conjugated on the spacer layer,
- wherein the fluorescent nanocomposite structure can be excited with light at λEX1 and can emit light at λEM2, and
- wherein an intensity of emission at λEM2 is greater than an intensity of emission at λEM1, and
- wherein the at least one fluorescent agent having λEX1 is a FRET donor to the at least one fluorescent agent having λEX2 which serves as a FRET acceptor.
Dependencies are inferred from the claim text.