Data acquisition and analysis method based on diabetes data analysis and processing equipment

A data acquisition and analysis method based on diabetes data analysis and processing equipment;

• the equipment comprises a computer, a placement rack and a flow cytometer, wherein the flow cytometer is mounted on the placement rack, and the computer is mounted on the placement rack;
• and characterized in that the data acquisition method comprises the following steps:
• step 1:
• cells acquisition:
• the white blood cells in peripheral venous blood of the patient are first acquired;
• the red blood cells are removed;
• then the peripheral venous blood cell suspension is added into an anticoagulant centrifuge tube to be centrifuged;
• and the supernatant after centrifugation is removed;
• step 2:
• cells processing:
• PBS solution is added to the liquid after preliminary processing in step 1 for washing and the washed liquid is centrifuged again;
• and then the supernatant after centrifugation is removed;
• step 3:
• cells reprocessing:
• the unit number of the white blood cells in the liquid after processing in step 2 is adjusted artificially;
• a probe is loaded in the adjusted liquid;
• and finally the cells are washed with serum-free culture solution;
• step 4:
• flow cytometer test:
• the cells are added to the flow cytometer, by which, the mitochondrial membrane potential in the white blood cells is tested;
• and
• step 5:
• independent evaluation and analysis of the neutrophil data are performed in the process of test.