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US 12,559,794 B2Granted
Method for nucleic acid amplification
AssigneeSt. Jude Children's Research Hospital
InventorsCharles Gawad, John Easton, Veronica Gonzalez-Pena
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Abstract
Provided herein are compositions and methods for accurate and scalable Primary Template-Directed Amplification (PTA) nucleic acid amplification and sequencing methods, and their applications for research, diagnostics, and treatment.
Claims30
A method of amplification comprising:
- (a) contacting at least one nucleic acid, at least one primer, a nucleic acid polymerase comprising 3′-5′ exonuclease activity, and a mixture of nucleotides, wherein the mixture of nucleotides comprises alpha-thio dideoxy nucleotide triphosphates;
- and
- (b) amplifying the at least one nucleic acid to generate a plurality of amplicons, wherein at least a portion of the plurality of amplicons comprises a 3′ terminal alpha-thio dideoxy nucleotide.
A method of amplification comprising:
- (a) contacting genomic DNA, at least one primer, a nucleic acid polymerase comprising 3′-5′ exonuclease activity, and a mixture of nucleotides, wherein the mixture of nucleotides comprises at least one terminator nucleotide that terminates nucleic acid replication by the polymerase;
- and
- (b) amplifying the genomic DNA to generate a plurality of amplicons, wherein at least a portion of the plurality of amplicons comprises a 3′ terminal terminator nucleotide.
Dependencies are inferred from the claim text.