Analyte enrichment methods and compositions
AssigneeQiagen Sciences, LLC
InventorsJerzy Olejnik, Steven Gordon, Martina Werner
Life of this patent
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Abstract
Methods and compositions for enriching a population of particles containing an analyte are disclosed. In one embodiment, enrichment beads are used that are larger in size than the beads used for amplification. A separation device is employed that can retain larger beads with bound amplified beads. The technique finds many uses, including enriching for beads with clonally amplified template, which can be used in a variety of assays, including nucleic acid sequencing.
Claims10
A population of emulsion bead—enrichment bead complexes captured upon a single layer nylon mesh, said emulsion beads comprising amplified template, wherein said amplified template is amplified from one nucleic acid template, wherein said emulsion beads are non-covalently bound to said enrichment beads and wherein at least 50% of said emulsion beads comprise homogeneous amplified template.
Dependencies are inferred from the claim text.